Etrebelle PN – PLLA + PN Hybrid Filler

Etrebelle PN logo

Synergistic effect of PLLA and PN (Polynucleotide) for collagenesis and tissue regeneration

PLLA uniform structure of spherical microparticles improves the safety and efficacy in volume restoration. Anti-inflammatory property of PN controls the foreign body reaction and promotes cell proliferation. Fundamental improvements from physical restoration to collagen production and tissue regeneration.

Etrebelle PN vial combining PLLA for volume restoration and collagenesis, HA for moisturizing and elasticity, and PN for anti-inflammation, tissue repair and growth factor secretion

PLLA microparticle structure

The uniform size (30-50µm) and structure of PLLA particles from the emulsification technique prevent nodule formation or injection needle/cannula clogging (27–32G). The smooth microsphere surface without microholes allows quick dilution and even distribution of the particles in the emulsion state.

Electron microscope images at 400x and 1000x: Etrebelle PN's PLLA particles are uniform smooth spheres, while products A and B show irregular fragments
Particle size distribution chart: Etrebelle PN shows a narrow, uniform peak below 100 µm compared with the broader distributions of products A and B
PN anti-inflammatory effect: inhibits inflammatory cytokines IL-6, TNF-α and HMGB1 and increases anti-inflammatory cytokine IL-10
PN activates growth factors EGF, FGF and IGF to promote fibroblast, osteoblast and chondrocyte growth, and VEGF for angiogenesis and improved microcirculation

PN synergistic effect

The physical restoration of PLLA particles under skin accompanies a foreign body reaction involving initial inflammatory responses. The included PN inhibits the inflammatory cytokine initially and promotes cell proliferation by activating essential growth factors. The mechanism contributes for collagenesis and angiogenesis effects eventually leading to the tissue regeneration. Not only with the physical stimulation of fibroblast (mechanotransduction), but the restoration property of PN shows the ideal synergistic effect simultaneously.

Etrebelle PN benefits: anti-inflammation, tissue regeneration, and boosted collagen production
Etrebelle PN right after injection: HA gel supports the area while PLLA and PN particles are distributed

After Injection

Physical Support

Under the skin, Etrebelle PN physically supports the injected area with its material properties, while non-cross-linked HA distributes the PLA particles internally. PN particles inhibit inflammatory responses from the foreign body reaction.

Etrebelle PN after 1 month: HA degrades as PN activates growth factors and new collagen fibers form

After 1 Month

Restoration

With the natural degradation of HA material, PLA particles evenly take position, resulting in the initial volumizing effects. PN particles initiate the activation of essential growth factors and stimulate fibroblasts.

Etrebelle PN after 6 months: PLLA particles integrate with new collagen fibers as PN supports cell proliferation

After 6 Months

Maintenance

PLA particles have now fully integrated with the skin tissue structure, inducing a collagenesis effect. PN particles contribute to cell proliferation through the secretion of growth factors.

Etrebelle after 24 months: microparticles fully eliminated, leaving newly formed collagen fibers

After 24 Months

Elimination

The PLA particles and naturally induced collagen are now indistinguishable. The promoted collagenesis effect and fibroblast stimulation enrich both extracellular components and the skin tissue environment.

Etrebelle PN vial: PLA + PN

Skin elasticity improvement, Fine wrinkle removal

Contouring facial line

Etrebelle PN

(PLA: 150mg + PN 35mg + HA: 15mg)

PLA particle: 30-50μm

PN Particle : 1,000 kDa

HA particle: 1,400kDa

Mixture: Sterile distilled water 6cc + Lidocaine 1cc

(dilution time: over 30min)

Cannula size: 27-32G

Etrebelle PN composition: PLA 150 mg and HA 1,400 kDa 15 mg